05-659
Anti-Kaiso Antibody, clone 6F
clone 6F, Upstate®, from mouse
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
6F, monoclonal
Speziesreaktivität
mouse, hamster, chicken, canine, rat, human, monkey
Hersteller/Markenname
Upstate®
Methode(n)
electrophoretic mobility shift assay: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG1
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... ZBTB33(10009)
Spezifität
Kaiso
Immunogen
Recombinant N-terminal GST and His-tagged fusion protein corresponding to residues 103-584 of murine Kaiso
Anwendung
Research Category
Epigenetik & nukleäre Funktionen
Epigenetik & nukleäre Funktionen
Research Sub Category
Transkriptionsfaktoren
Transkriptionsfaktoren
Detect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC.
Qualität
Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells.
Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999).
Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001).
Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).
Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999).
Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001).
Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).
Zielbeschreibung
97kDa
Physikalische Form
Protein G Chromatography
0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.
Format: Purified
Lagerung und Haltbarkeit
2 years at -20°C
Hinweis zur Analyse
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Rechtliche Hinweise
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
J M Daniel et al.
Hybridoma, 20(3), 159-166 (2001-07-20)
The POZ-zinc finger protein Kaiso belongs to a rapidly growing superfamily of BTB/POZ zinc finger transcription factors implicated in embryonic development and cancer. Kaiso interacts with the catenin p120(ctn), but the significance of the interaction remains unknown. Although p120(ctn) is
Shun-Dong Dai et al.
Cancer science, 102(1), 95-103 (2010-11-13)
δ-Catenin is the only member of the p120 catenin (p120ctn) subfamily that its primary expression is restricted to the brain. Since δ-catenin is upregulated in human lung cancer, the effects of δ-catenin overexpression in lung cancer still need to be
A Prokhortchouk et al.
Genes & development, 15(13), 1613-1618 (2001-07-11)
We describe a novel mammalian DNA binding activity that requires at least two symmetrically methylated CpG dinucleotides in its recognition sequence, preferably within the sequence 5'CGCG. A key component of the activity is Kaiso, a protein with POZ and zinc-finger
The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor.
J M Daniel et al.
Molecular and cellular biology, 19(5), 3614-3623 (1999-04-17)
p120(ctn) is an Armadillo repeat domain protein with structural similarity to the cell adhesion cofactors beta-catenin and plakoglobin. All three proteins interact directly with the cytoplasmic domain of the transmembrane cell adhesion molecule E-cadherin; beta-catenin and plakoglobin bind a carboxy-terminal
Shun-Dong Dai et al.
BMC cancer, 9, 178-178 (2009-06-11)
Kaiso has been identified as a new member of the POZ-zinc finger family of transcription factors that are implicated in development and cancer. Although controversy still exists, Kaiso is supposed to be involved in human cancer. However, there is limited
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