Direkt zum Inhalt
Merck
Alle Fotos(1)

Wichtige Dokumente

05-1579

Sigma-Aldrich

Anti-Axin-1 Antibody, clone A5

clone A5, from mouse

Synonym(e):

Axis inhibition protein 1, axin 1, axis inhibitor 1, fused, mouse, homolog of

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

A5, monoclonal

Speziesreaktivität

human

Speziesreaktivität (Voraussage durch Homologie)

mouse (based on 100% sequence homology)

Methode(n)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG2aκ

NCBI-Hinterlegungsnummer

NP_033863.2

UniProt-Hinterlegungsnummer

O35625

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... AXIN1(8312)
mouse ... Axin1(12005)

Allgemeine Beschreibung

Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.

Spezifität

This antibody recognizes the N-terminus of Axin-1.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.

Anwendung

Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.

Qualität

Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.

Zielbeschreibung

~ 97 kDa

Physikalische Form

Format: Purified

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sie haben nicht das passende Produkt gefunden?  

Probieren Sie unser Produkt-Auswahlhilfe. aus.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Phosphatidylinositol 3-kinase signaling does not activate the wnt cascade.
Ng, SS; Mahmoudi, T; Danenberg, E; Bejaoui, I; de Lau, W; Korswagen, HC; Schutte, M; Clevers, H
The Journal of Biological Chemistry null
Chuifeng Fan et al.
Molecular carcinogenesis, 54 Suppl 1, E138-E147 (2014-09-30)
Our previous studies indicate that abnormal expression of several Wnt signaling molecules including Axin, Dvl and β-catenin are involved in proliferation, invasion and metastasis of lung cancer. Zbed3 was found to inhibit function of Axin-GSK3β complex and thus lead to
Rong Xie et al.
eLife, 11 (2022-12-22)
Axin1 is a key regulator of canonical Wnt signaling pathway. Roles of Axin1 in skeletal development and in disease occurrence have not been fully defined. Here, we report that Axin1 is essential for lower limb development. Specific deletion of Axin1
S Monteagudo et al.
Osteoarthritis and cartilage, 30(5), 724-734 (2022-03-02)
To investigate how ANP32A, previously linked to the antioxidant response, regulates Wnt signaling as unraveled by transcriptome analysis of Anp32a-deficient mouse articular cartilage, and its implications for osteoarthritis (OA) and diseases beyond the joint. Anp32a knockdown chondrogenic ATDC5 cells were
Eunsun Kim et al.
Nature communications, 10(1), 1909-1909 (2019-04-25)
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. β-catenin is widely thought to be a major oncogene in HCC based on the frequency of mutations associated with aberrant Wnt signaling in HCC patients. Challenging this model
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.