03-32-5032

MMP-2/MMP-7 Substrate, Fluorogenic

Extremely sensitive fluorogenic substrate for matrix metalloproteinases (MMPs).

• Synonym(e): MMP-2/MMP-7 Substrate, Fluorogenic
• Empirische Formel (Hill-System): C₄₉H₆₈N₁₄O₁₅
• Molekulargewicht: 1093.15
• UNSPSC-Code: 12352209
• NACRES: NA.83

Eigenschaften

• Qualitätsniveau: 200
• Assay: ≥95% (HPLC)
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; desiccated
• Farbe: yellow
• Löslichkeit: 20% acetic acid: 1 mg/mL
• Fluoreszenz: λ_ex ~325 nm; λ_em ~393 nm
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Extremely sensitive fluorogenic substrate for matrix metalloproteinases (MMPs). Suitable for assessment of total activity in crude preparations by continuous assays (k_cat/K_m = 1.7 x 10⁵ M^-1sec^-1 for MMP-7 and 6.3 x 10⁵ M^-1sec^-1 for MMP-2).

Biochem./physiol. Wirkung

Cell permeable: no

Primary Target
Fluorogenic substrate for matrix metalloproteinases (MMPs)

Product does not compete with ATP.

Reversible: no

kcat/Km = 1.7 x 105 M-1sec-1 for MMP-7 and 6.3 x 105 M⁻¹ sec⁻¹ for MMP-2

Warnhinweis

Toxicity: Standard Handling (A)

Sequenz

MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ ·TFA [Dpa = N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]

Physikalische Form

Supplied as a trifluoroacetate salt.

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sonstige Hinweise

Knight, C.G., et al. 1992. FEBS Lett. 296, 263.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable