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HPLC Analysis of PAHs on SUPELCOSIL™ LC-PAH

HPLC Analysis of PAHs on SUPELCOSIL™ LC-PAH application for HPLC

Materials

analytical column

Product No.
Description
Pricing

58318

SUPELCOSIL™ LC-PAH HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

SUPELCOSIL LC-PAH, 15 cm x 4.6 mm I.D., 5 μm particles (58318)

mobile phase

[A] water, [B] methanol

gradient

55 to 100% B in 5 min; held at 100%B for 10 min

flow rate

1.5 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

5 μL

sample

EPA 610 mix

Description

Analysis Note

PAHs are traditionally evaluated using an acetonitrile/water gradient. Recently customers are looking for alternatives to this method using a methanol/water gradient due to the risk of an another acetonitrile shortage, solvent costs, and toxicity to the environment. This analysis demostrates the separation using a methanol gradient.

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS