Solid Phase Micro-Extraction (SPME)
Solid phase microextraction (SPME) is a versatile and solvent-free sample preparation technique. It works on the principle of adsorption/absorption and desorption and uses a fiber coated with an extractive phase to concentrate analytes in a sample. It successfully combines the sampling, isolation, and enrichment of an analyte to one step. SPME is used widely for the sampling of environmental volatiles, food & beverages, flavors & fragrances, forensics & toxicology, and pharmaceutical samples.
Related Product Resources
- Selection Guide for Supelco SPME Fibers
Recommended fibers based on analyte type and molecular weight; tips for SPME fiber selection for a variety of applications.
- The use of SPME-GC/MS to determine the best packaging material for preventing light-induced off-flavors in milk
yethylene (HDPE) plastic jugs instead of glass bottles. In this study, we used solid-phase microextraction (SPME) followed by GC/MS to investigate the effectiveness of a range of plastic materials as barriers to the production of offflavors.
- Rugged and Reproducible Solid Phase Microextraction (SPME) Sampling
SPME fibers with Carboxen®/Polydimethylsiloxane (CAR/PDMS) and Polydimethylsiloxane/ Divinylbenzene (PDMS/DVB) coatings are now available on a nitinol core (NIT) in a SS assembly for improved fiber performance.
- Brochure: SPME for GC Analysis
Solid Phase Microextraction (SPME) is an innovative, solvent-free sample prep technology that is fast, economical, and versatile.
- Brochure: Supel™ BioSPME 96-Pin Devices
Supel™ BioSPME 96-Pin Devices were developed to target the stringent specifications needed by bioanalytical laboratories, yet greatly improve upon the speed and simplicity compared with current sample preparation techniques employed for plasma protein binding studies today.
- Application Note: Sample Preparation of Serum Prior to Determination of Free Testosterone
The industry’s gold standard for sample preparation of free hormones from serum has been associated with equilibrium dialysis.
- Protein Binding Determination - Comparison Study of Techniques & Devices
Comparative analysis of Supel™ BioSPME 96-Pin device with a rapid equilibrium dialysis technique for accuracy of measured values, sample cleanliness, and workflow time in drug protein binding.
Smart SPME fibers
The smart SPME fibers are an extension of our expertise and innovation from traditional SPME coatings to smart SPME format for accurate, reliable, and consistent results. Our smart fibers including Carboxen® fibers, dual coated fibers, or overcoated fibers are fitted with a unique chip to offer seamless sample preparation and other including benefits:
- Traceability to check usage, stroke count, dates of operation, and temperature exposure
- High-throughput sample preparation
- Automatic application of correct parameters for each fiber coating
See for more information on Smart SPME Fibers for Automated Sampling.
nitinol-core SPME fibers
The nitinol-core of SPME fibers enhances the fiber performance and the precision of chromatographic results. Fibers with either Carboxen®/Polydimethylsiloxane (CAR/PDMS) or Polydimethylsiloxane/ Divinylbenzene (PDMS/DVB) coatings are available with a nitinol-core. Other benefits of nitinol fibers include:
- Enhanced lot-to-lot and fiber-to-fiber reproducibility
- Higher fiber durability
- Similar selectivity and inertness compared to fused silica core fibers
See for more information on Nitinol Core SPME Fibers.
OVERCOATED (OC) SPME FIBERS
The over coating of fibers is a new technology in fiber coating to protect the SPME fiber from matrix components. This allows for immersion of the fiber directly into complex samples ensuring a more efficient extraction (e.g. of semivolatile compounds). Polydimethylsiloxane (PDMS) is used to overcoat a PDMS/divinylbenzene (DVB) fiber. The resulting fiber is more physically robust and resistant to chemical fouling and allows for an efficient wash step to also remove adhering matrix.
See Overcoated Fibers available.
BioSPME
BioSPME technology greatly improves upon the speed and simplicity compared with current bioanalytical sample preparation techniques employed for plasma protein binding studies and other free analyte analyses. The primary benefits of Supel™ BioSPME devices include:
- 3x faster workflow compared to rapid equilibrium dialysis based methods
- Universal 96-pin format, compatible with standard consumables
- Simple workflow amenable to total automation or manual operation
See for more information on Supel™ BioSPME 96-Pin Devices.
Related Webinars
To continue reading please sign in or create an account.
Don't Have An Account?