GC Analysis of a 38-Component FAME Mix on SLB®-IL111, 200 m Column
Materials
analytical column
CONDITIONS
column
SLB-IL111, 200 m x 0.25 mm I.D., 0.20 μm (29689-U)
oven
100 °C (13 min), 4 °C/min to 240 °C (30 min)
inj. temp.
250 °C
detector
FID, 250 °C
carrier gas
hydrogen, 27 cm/sec
injection
1.0 μL, 50:1 split
liner
4 mm I.D., split type, cup design
sample
Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME
Description
Analysis Note
Identification and quantitation of individual fatty acid isomers in complex mixtures of isomers is desirable for both food and clinical scientists. This application requires the fatty acids to be converted to fatty acid methyl esters (FAMEs), followed by analysis using a long, efficient GC column with a stationary phase highly selective for polarizable analytes (those with double and/or triple C-C bonds). A 200 m SLB-IL111 is such a column.
Legal Information
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany