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  • A selective LC/RIA for dexamethasone and its prodrug dexamethasone-21-isonicotinate in biological fluids.

A selective LC/RIA for dexamethasone and its prodrug dexamethasone-21-isonicotinate in biological fluids.

Journal of pharmaceutical and biomedical analysis (1991-01-01)
G Hochhaus, H Derendorf, H Möllmann, J Barth
ABSTRACT

A combined LC/RIA procedure is described for the selective determination of dexamethasone (DEX) and its prodrug dexamethasone-21-isonicotinate (DIN) in plasma. The low affinity of the employed dexamethasone antiserum for DIN (cross-reactivity less than 0.5%) allowed the direct determination of DEX in plasma extracts. For the determination of DIN, both substances of interest were separated by LC, the DIN containing fraction was collected, hydrolysed and the generated DEX was consequently assayed by radioimmunoassay. The assay detection limits were 0.1 ng ml-1 for DEX and 0.75 ng ml-1 for DIN. For both substances, inter- and intra-day variabilities (RSDs) were 6 and 12%, respectively.

MATERIALS
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Product Description

Dexamethasone isonicotinate for impurity C identification, European Pharmacopoeia (EP) Reference Standard
Pricing and availability is not currently available.
Dexamethasone isonicotinate, European Pharmacopoeia (EP) Reference Standard
Pricing and availability is not currently available.