ZRB1132

Anti-VCAM-1 Antibody, clone 5K19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5K19, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 81.28 kDa; observed mol wt ~110 kDa
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001069.1
• UniProt accession no.: P19320
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 5K19 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Vascular cell adhesion protein 1 (VCAM-1). It targets an epitope within 17 amino acids from the C-terminal, extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal, extracellular domain of human Vascular cell adhesion protein 1 (VCAM-1).

Application

Quality Control Testing

Evaluated by Western Blotting with human recombinant VCAM-1.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected human recombinant VCAM-1.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected VCAM-1 in C2C12, NIH3T3, HUT-78, and MCF-7 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound VCAM-1 peptide with a KD of 5.6 x 10-6 in an affinity binding assay.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected VCAM-1 in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VCAM-1 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-VCAM-1, clone 5K19 ZooMAb^®, Cat. No. ZRB1132, is a recombinant Mouse monoclonal antibody that specifically targets VCAM-1 and is tested for use in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Vascular cell adhesion protein 1 (UniProt: P19320; also known as V-CAM 1, VCAM-1, INCAM-100, CD106) is encoded by the VCAM1 gene (Gene ID: 7412) in human. VCAM-1 is a single-pass type I membrane glycoprotein that is predominantly expressed in endothelial cells. It is reported to be absent on resting endothelial cells, but the cells respond to IL-1 and TNFa by upregulating the expression of VCAM-1, with maximal activity reaching at 6 to 12 hours. VCAM-1 functions in leukocyte-endothelial cell adhesion. It interacts with integrin a4/b1 on leukocytes and mediates both adhesion and signal transduction. Under high degree of inflammation and chronic conditions in some diseases, VCAM-1 expression is also expressed on the surface of other cells, including tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts, oocytes, Kupffer cells, Sertoli cells, and cancer cells. VCAM-1 is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-698), a transmembrane domain (aa 699-720), and a cytoplasmic domain (aa 721-739). The extracellular domain contains seven Ig-like C2-type domains. Ig-like domain pairs of 1 and 4, 2 and 5, and 3 and 6 are highly homologous with each other. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kong, DH., et al. (2018). Int. J. Mol. Sci. 19(4); 1057).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable