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Z764000

MicroTissues® 3D Petri Dish® micro-mold spheroids

size S, 16 x 16 array, fits 12 well plates

Synonym(s):

3D, 3D Cell Culture

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About This Item

UNSPSC Code:
41121812
NACRES:
NB.14
Pricing and availability is not currently available.

material

spherical

size

S

sterility

sterile; autoclaved

feature

lid: no
 16 x 16 array

packaging

pack of 6 ea

manufacturer/tradename

MicroTissues Inc. 12-256

volume

190 μL

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General description

Six autoclavable precision micro-molds to cast 3D Petri Dish for forming small spheroids. 3D Petri Dish for use in 12-well plate. Each micro-mold forms 256 circular recesses in a 16 x 16 array.

  • Nominal dimensions of each 3D culture recess: diam. 300 μm x D 800 μm
  • Micro-molds also form single chamber for cell seeding
When the gelled agarose is removed from the micro-mold, it is transferred to a standard 12 well or 24 well tissue culture dish and equilibrated with cell culture medium.

Since the agarose is transparent, the spheroids or microtissues that form at the bottom of each agarose micro-well can be easily viewed using a standard inverted microscope using phase contrast, bright field or fluorescence microscopy.

  • The micro-molds are reusable up to 12 times and can be sterilized via a standard steam autoclave (30 min, dry cycle)
  • The micro-molds should be stored in a covered container to maintain sterility and to avoid collecting dust or fibres on their small features
  • Any microscope with sufficient magnification and resolution functions for cell based views or images will be suitable for use with the 3D Petri Dish products

Legal Information

3D Petri Dish is a registered trademark of MicroTissues Inc.
MicroTissues is a registered trademark of MicroTissues Inc.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Minjae Kim et al.
Journal of cell science, 132(19) (2019-09-08)
Cultured rat primitive extraembryonic endoderm (pXEN) cells easily form free-floating multicellular vesicles de novo, exemplifying a poorly studied type of morphogenesis. Here, we reveal the underlying mechanism and the identity of the vesicles. We resolve the morphogenesis into vacuolization, vesiculation

Questions

  1. What are the dimensions of the 16 x 16 array (i.e., x mm by y mm) (fits 12 well, S), 9x9 array (fits 12 well, L), 8x12 array (24 well plate, S), and 5x7 array (24 well plate, L)?

    1 answer

    1. The 3D Petri Dish products are flexible molds intended for use as a vessel to form 3D gels for culture use. The resulting gels meet the following specifications as provided by the manufacturer, MicroTissues. Inc. In addition, please see the images below for representative examples of this principal.

      16 x 16-The nominal dimensions of one of these recesses when formed in the 3D Petri Dish® are diameter: 0.3mm and depth: 0.8mm The single large cell seeding chamber above these 256 recesses holds a volume of 190μl. This 3D Petri Dish® fits into a standard 12 well tissue culture plate. (6 micro-molds per pack)
      https://www.microtissues.com/buy-now/12-256-small-spheroids

      8 x 12-The nominal dimensions of one of these recesses when formed in the 3D Petri Dish® are; diameter: 0.4mm and depth: 0.8mm. The single large cell seeding chamber above these 96 recesses holds a volume of 75μl. The 3D Petri Dish® fits into a standard 24 well tissue culture plate. (6 micro-molds per pack)
      https://www.microtissues.com/buy-now/24-96-small-spheroids

      9x9-The nominal dimensions of one of these recesses when formed in the 3D Petri Dish® are; diameter: 0.8mm and depth: 0.8mm. The single large cell seeding chamber above these 81 recesses holds a volume of 190μl. This 3D Petri Dish® fits into a standard 12 well tissue culture plate. (6 micro-molds per pack)
      https://www.microtissues.com/buy-now/12-81-large-spheroids

      5x7-The nominal dimensions of one of these recesses when formed in the 3D Petri Dish® are; diameter: 0.8mm and depth: 0.8mm. The single large cell seeding chamber above these 35 recesses holds a volume of 75μl. This 3D Petri Dish® fits into a standard 24 well tissue culture plate. (6 micro-molds per pack)
      https://www.microtissues.com/buy-now/24-35-large-spheroids

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