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X2753

Sigma-Aldrich

Xylanase

greener alternative

powder, ≥2500 units/g, recombinant, expressed in Aspergillus oryzae

Synonym(s):

Pentopan Mono BG®

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About This Item

CAS Number:
37278-89-0
EC Number:
253-439-7
MDL number:
MFCD00132594
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

recombinant

expressed in Aspergillus oryzae

Quality Level

200

form

powder

specific activity

≥2500 units/g

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Enabling,

storage temp.

2-8°C

General description

Purified endo-β−(1→4)-xylanase from Thermomyces lanuginosus produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism
Studies show that the signal sequence found in the xylanase gene is critical for protein production in E. coli. Loss of this sequence resulted in a significant reduction in expression. [1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research
Xylanase catalyses the degradation of xylan into xylooligosaccharides and D-xylose. It also breaks down hemicellulose. Xylanase is used for the production of biofuels, such as ethanol and xylitol. It improves the quality of bread and juices.[2] Xylanase is used in biobleaching process and decreases environmental pollution.[3]

Application

Xylanase has been used:
  • in the isolation of hemicellulose and production of oligosaccharides[4]
  • in viscosimetric assay[5]
  • in the comparison of commercial enzymes for the aqueous enzymatic extraction of corn oil from corn germ[6]
  • in the hydrolytic degradation of xylan[7]

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Legal Information

A product of Novozyme Corp.
Pentopan is a registered trademark of Novozymes Corp.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000374140
0000374140
0000340680
0000340681
0000287585

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Svini Dileepa Marasinghe et al.
Microbial cell factories, 20(1), 129-129 (2021-07-10)
Xylanase-containing enzyme cocktails are used on an industrial scale to convert xylan into value-added products, as they hydrolyse the β-1,4-glycosidic linkages between xylopyranosyl residues. In the present study, we focused on xynS1, the glycoside hydrolase (GH) 11 xylanase gene derived
Gautam Anand et al.
International journal of molecular sciences, 22(8) (2021-05-01)
Plants lack a circulating adaptive immune system to protect themselves against pathogens. Therefore, they have evolved an innate immune system based upon complicated and efficient defense mechanisms, either constitutive or inducible. Plant defense responses are triggered by elicitors such as
Polarized-Light-Stimulated Enzymatic Hydrolysis of Xylan
Konieczna-Molenda A, et al.
Biotechnology Progress, 24(2), 385-388 (2008)
XYLANASES AND THEIR INDUSTRIAL APPLICATIONS: A REVIEW
Kumar D, et al.
Biochemical and Cellular Archives, 17(1), 353-360 (2017)
Microbial xylanases and their industrial application in pulp and paper biobleaching: a review
Walia A, et al.
3 Biotech, 7(1), 11-11 (2017)
View All Related Papers

Questions

1–7 of 7 Questions  

  1. Will this enzyme in buffer be deactivated completely at 100°C boiling for 10 mins towards end of protocol or it needs acid treatment strictly ?

    1 answer

    1. We have not tested boiling this enzyme at the end of the protocol, we have only used acid.

      Helpful?

  2. Is this enzyme powder soluble in water ? seems like particle dispersing in buffer at Ph 4.8 ?

    1 answer

    1. The active components of xylanase X2753 are readily soluble in water at concentrations that occur in normal usage. However, water solutions will be turbid because of the wheat flour used for standardization of the enzyme.

      Helpful?

  3. What does >2500U/g imply. What is range of enzyme activity for this enzyme under your protocols kindly inform.

    1 answer

    1. Each Lot is guaranteed to have a specific activity greater than or equal to 2500 Units/g. There is no maximal value specified.
      The calculated specific activity for each lot will be printed on the Certificate of Analysis found here:
      https://www.sigmaaldrich.com/product/sigma/x2753#product-documentation

      Helpful?

  4. What is the optimal pH, buffer, temperature and concentration of this product ?

    1 answer

    1. This is a product of Novozymes Corp. The manufacturer tests this material according to the following protocol and conditions:
      Temperature - 50°C
      Buffer - 0.1M Phosphate Buffer, pH 6
      Enzyme concentration - 0.007 - 0.057 units/mL
      Substrate - 0.45%w/v Azo-Wheat arabinxylan in 0.1 M Phosphate Buffer, pH 6
      Reaction - 0.100 mL enzyme solution added to 0.900 mL substrate solution
      Incubation time - 30 minutes in a water bath at 50°C
      Stop Reaction - add 5 mL of Stop Reagent: 6.66 mL 2 N HCl in 1000mL absolute Ethanol
      Incubation - 15 - 60 minutes at ambient temperature
      Centrifuge at 4000 rmp for 15 minutes
      Detection - 585 nm (manual) within 20 minutes of centrifugation

      Helpful?

  5. We have Sigma X2753 Xylanase We need to treat our sample with this. Kindly suggest a protocol at which this product will show its optimum activity.

    1 answer

    1. There is not a protocol offered specifically for this product. However, the peer-reviewed articles may be helpful:
      https://www.sigmaaldrich.com/search/x2753?focus=papers&page=1&perpage=30&sort=relevance&term=x2753&type=citation_search

      Helpful?

  6. What is the density of Product X2753, Cellulase from Aspergillus oryzae?

    1 answer

    1. We have not determined the density of this product.

      Helpful?

  7. What is the optimum pH for Cellulase from Aspergillus oryzae., Product X2753?

    1 answer

    1. Product X2753, Xylanase, has activity in the pH range of 4-6, and tolerance with temperatures up to 75 °C. The following article may be helpful: https://www.sciencedirect.com/science/article/pii/S138917230670603X#aep-section-id10

      Helpful?

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