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WG16

Sigma-Aldrich

Wright-Giemsa Stain, Modified

Wright-Giemsa Stain, Modified
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About This Item

MDL number:
UNSPSC Code:
41116121
eCl@ss:
42039003
NACRES:
NA.47
Pricing and availability is not currently available.

form

solution

Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

concentration

0.4 % (w/v) in methanol (buffered at pH 6.8)

pH

6.7-6.9

application(s)

hematology
histology

storage temp.

room temp

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Application

When blood films are stained using Wright-Giemsa Stain, the white blood cell nucleus and cytoplasm take on the characteristic blue or pink coloration. The combination of purified eosin and thiazine dyes in the product eliminates inconsistent staining and yields reproducible chromogenic responses.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

51.8 °F - closed cup

Flash Point(C)

11 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number

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Questions

  1. What is the preservation process for slides stained with the Wright-Giemsa stain if they cannot be viewed immediately?

    1 answer
    1. the Wright Giemsa Modified solution is intended for staining peripheral blood or bone marrow smears, without mention of staining tissue sections. If staining smears, simply complete the staining procedure as directed in the IFU, then store the dried slides in a slide folder or box without coverslipping, as coverslipping is not recommended and is not a common practice in hospital settings.

      For long-term storage, slides are typically stored in slide file cabinets, and oil immersion is usually not required. However, if slides need to be viewed under oil immersion, a coverslip can be placed atop the stained slide, and once viewed, the coverslip can be removed and discarded. Any excess oil can be absorbed using a Kimwipe or tissue paper without wiping, as wiping may remove stained cells from the slide.

      Although no formal studies have been conducted to determine the longevity of stained slides, it is expected that the staining should be adequate for several weeks or months. For longer retention, it is suggested to take pictures of several fields from the slides for archival purposes.

      Helpful?

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