SRP6251

CCL6 from mouse

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): C10, CCL6, MRP-1, SCYA6
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: calculated mol wt 11.4 kDa (The predicted N-terminus is Gly22.); observed mol wt 14 kDa by SDS-PAGE (reducing)
• packaging: pkg of 10 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P27784
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... CCL6(20305)

Description

General description

Chemokine (C-C motif) ligand 6 (CCL6), a member of the CC chemokine family, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. CCL6 has only been identified in rodents. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, where it is strongly induced upon GM-CSF stimulation. Mouse CCL6 expression was also shown to be strongly induced by interleukin-4 (IL-4) in mouse macrophages and by cytokine IL-13 in mouse lung. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable