SRP0183
Hsp90b Active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
HSP84, HSP90Beta, HSPC2, Heat shock protein 90 kDa β
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.
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biological source
human
recombinant
expressed in E. coli
Assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
83 kDa
packaging
pkg of 200 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... HSP90AB1(3326)
General description
Hsp90b (heat shock protein 90-β) is a constitutive cytosolic isoform of Hsp90.[1] The gene is mapped to human chromosome 6p21.1.[2] Hsp90b is an ATP-dependent molecular chaperone and is upregulated in many tumor cells.[3]
Human Heat Shock Protein 90β (GenBank Accession No. AY359878), full length with C-terminal His tag, MW = 83kDa, expressed in an Escherichia coli expression system.
Human Heat Shock Protein 90β (GenBank Accession No. AY359878), full length with C-terminal His tag, MW = 83kDa, expressed in an Escherichia coli expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Hsp90 (heat shock protein 90) is needed for folding, maturation, transport, and degradation of various proteins, particularly signaling molecules, steroid receptors, and transcription factors. Hsp90b (Hsp90 β) is secreted by various cells and works as a chaperone for selected surface-associated or secreted proteins. It acts as a chaperone for matrix metalloproteinases and extracellular matrix proteins, and helps in cell motility.[1] Suppression of the Hsp90b activity is suggested as a therapeutic approach for various cancers and inflammatory diseases.[4]
Physical form
Formulated in 25 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
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HSP90AB1: Helping the good and the bad.
Haase M, et al.
Gene, 575, 171-171 (2016)
Cell surface heparan sulfate proteoglycans are involved in the binding of Hsp90a and Hsp90? to the cell plasma membrane.
Snigireva AV, et al.
Cell adhesion & migration, 9, 460-460 (2015)
[Hsp90AB1 Protein is Overexpressed in Non-small Cell Lung Cancer Tissues ?and Associated with Poor Prognosis in Lung Adenocarcinoma Patients].
Wang M, et al.
Zhongguo Fei ai za Zhi = Chinese Journal of Lung Cancer, 19, 64-64 (2016)
DNA copy number analysis of Grade II-III and Grade IV gliomas reveals differences in molecular ontogeny including chromothripsis associated with IDH mutation status.
Cohen A, et al.
Acta Neuropathologica Communications, 3, 34-34 (2015)
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