SRP0123

JMJD2C Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonym(s): GASC1, Gene amplified in squamous cell carcinoma 1 protein, JHDM3C, KIAA0780, Lysine-specific demethylase 4C (KDM4C)
• UNSPSC Code: 12352200

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• mol wt: 69 kDa
• packaging: pkg of 100 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: BC143571
• UniProt accession no.: Q9H3R0
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... KDM4C(23081)

Description

General description

JMJD2C (Jumonji domain containing), also known as KDM4C (lysine demethylase 4C), is an H3K9me3 demethylase belonging to the conserved family of H3K9me3 erasers, KDM4 demethylases. KDM4 family was first identified in 2006, and is composed of six members namely KDM4A, -B, -C, -D, -E, and -F. This protein predominantly resides at H3K4me3-containing promoter region. JMJD2C protein has multiple reader domains, including a hybrid tandem tudor domain (TTD) and two PHDs (plant homeodomain) of unknown function.
Human JMJD2C, GenBank Accession No. BC143571) (amino-acids 2-372) with N-terminal ST tag, MW = 69kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

JMJD2C (Jumonji domain containing), also known as KDM4C (lysine demethylase 4C), is a type of KDM4 demethylases that are responsible for the demethylation of H3K9me2/3, H3K36me3/2, and H1.4K26me3/2. This is accomplished through a hydroxylation reaction requiring the cofactors Fe(II) and 2-oxoglutarate (2-OG). These proteins show heightened expression and activity in multiple types of cancers, and hence, are also classified as oncogenes. KDMA4 family members are capable of coregulating hormone receptors, and hence, are implicated in the progression of hormone dependent cancers, such as breast and prostate cancer. JMJD2C is essential for regulating the expression of growth promoting proteins and the survival factor FRA1 (Fos-related antigen 1) and cyclin D1, and BCL2 (B-cell lymphoma) respectively. The expression of JMJD2C is increased in colon cancer cell, where it interacts with the oncogenic protein β-catenin, the up-regulation of which is essential for the pathogenesis of colon cancer.

Unit Definition

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.