SAB4300670

Anti-phospho-SP1 (pThr⁷³⁹) antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-Sp1 transcription factor, Anti-TSFP1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~90 kDa
• species reactivity: human, mouse, rat
• concentration: 1 mg/mL
• technique(s): western blot: 1:500-1:1000
• isotype: IgG
• immunogen sequence: (T-A-Tp-P-S)
• NCBI accession no.: NM_138473.2
• UniProt accession no.: P08047
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pThr739)
• Gene Information: human ... SP1(6667)

Description

General description

Specific protein 1 (Sp1) is a transcription regulator that has a transactivation domain at the N-terminus and a DNA-binding domain at the C-terminus, that contain three zinc fingers (ZF). It belongs to transcription factor family and is located on chromosome 12q13.

Immunogen

Peptide sequence around phosphorylation site of threonine 739 (T-A-Tp-P-S), according to the protein NP_612482.2

Application

Anti-phospho-SP1 (pThr739) antibody has been used in immunohistochemistry.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Specific protein 1 (Sp1) controls the up- and down-regulation of hCtr1 (human high-affinity copper transporter 1). It controls the expression of several genes that participates in differentiation, cell cycle and oncogenesis. It has the ability to bind GC/GT‐rich promoter elements with the help of C(2)H(2)‐type zinc fingers at the C‐terminal domain.

Features and Benefits

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Target description

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable