SAB4200270

Anti-PSMB9 antibody, Mouse monoclonal

clone LMP2-37, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-LMP2, Monoclonal Anti-PSMB6i, Monoclonal Anti-RING12, Monoclonal Anti-beta1i, Monoclonal Anti-proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: LMP2-37, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 17 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1.0-2.0 μg/mL using extracts of rat spleen
• isotype: IgG2a
• UniProt accession no.: P28065
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PSMB9(5698)

Description

General description

Monoclonal Anti-PSMB9 (mouse IgG2a isotype) is derived from the hybridoma LMP2-37 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human PSMB9 recombinant protein. Proteasome 20S subunit beta 9 (PSMB9) is present in the major histocompatibility complex (MHC) class II region. γ-interferon triggers the synthesis of three proteasomal subunits-PSMB9 (β1i), PSMB10 (β2i) and PSMB8 (β5i), which is a part of immunoproteasome.

Immunogen

human PSMB9, recombinant protein.

Application

Monoclonal Anti-PSMB9 antibody produced in mouse has been used in:

• flow cytometry
• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

Proteasome 20S subunit beta 9 (PSMB9) is used during antigen presentation. Aberrant levels of PSMB9 is involved in autoimmune disorders such as primary Sjogren′s syndrome and uterine leiomyosarcomas. The proteasome is a multicatalytic proteinase complex, which is involved in the regulation of essential cellular processes such as transcription, cell cycle progression, differentiation, apoptosis and in the control of stress and immune responses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable