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RAB1590

Sigma-Aldrich

Human NELL-1 ELISA

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/ml

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NELL1(4745)

General description

The NELL-1 (neural EGFL like 1) gene encodes an extracellular glycoprotein and is mapped to human chromosome 11p15. Nell-1 is a 810 amino acids protein containing thrombospondin-1 module at its N-terminus and includes other motifs : five von Willebrand factor C domains and six epidermal growth factor (EGF)-like domains.
This ELISA antibody pair detects human NELL-1. Other species are not determined.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.

Biochem/physiol Actions

NELL-1 (neural EGFL like 1) is associated with the risk of Rhabdomyosarcomas and the gene is identified to be hypomethylated. The protein encoded influences osteogenic related processes and thus loss of the NELL-1 gene function leads to abnormality in bone and cartilage. NELL-1 is considered as a essential factor in skeletal development as it controls the action of runt-related transcription factor 2. Lack of Nell-1 protein decreases the expression of gene encoding cartilage proteins such as collagens, tenascins, matrilins, and chondroadherin. It serves as an important growth factor in promoting regeneration of articular cartilage. NELL-1 is a tumor suppressor gene and hypermethylation of its promoter region indicates poor prognosis of the disease in esophageal adenocarcinoma.

Other Notes

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Accelerated chondrogenic differentiation of human perivascular stem cells with NELL-1.
Li C S, et al.
Tissue Engineering: Part A, 22(3-4), 272-285 (2016)
NELL1, whose high expression correlates with negative outcomes, has different methylation patterns in alveolar and embryonal rhabdomyosarcoma.
Tombolan L, et al.
Oncotarget, 8(20), 33086-33086 (2017)
Novel Wnt regulator NEL-like molecule-1 antagonizes adipogenesis and augments osteogenesis induced by bone morphogenetic protein 2.
Shen J, et al.
The American Journal of Pathology, 186(2), 419-434 (2016)
MALDI-TOF mass array analysis of Nell-1 promoter methylation patterns in human gastric cancer.
Gao C, et al.
BioMed Research International, 2015 (2015)
Ritsuko Nakamura et al.
Cancer science, 106(5), 656-664 (2015-03-03)
Neural epidermal growth factor-like like (NELL) 1 and 2 constitute a family of multimeric and multimodular extracellular glycoproteins. Although the osteogenic effects of NELL1 and functions of NELL2 in neural development have been reported, their expression and functions in cancer
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