Ceramic HyperD media are used in protein chromatography and affinity chromatography. Ceramic HyperD media have been used in the development of a capture step of a human recombinant F(ab′)(2) produced and expressed in baculovirus-infected cells and to identify whey proteins as a model system for chromatographic separation of proteins.
Features and Benefits
HyperD media are highly porous, rigid ceramic beads filled with a functionalized hydrophilic gel. The rigid bead allows extremely high linear flow rates without bed compression. The hydrogel exchanges throughout its volume, not just on the surface. And the media do not change volume due to changes in pH or ionic strength. The supports are stable in commonly used solvents and alkaline or acid environments. Supplied as aqueous suspensions in 1 M NaCl with 20% ethanol to inhibit bacterial growth.
Legal Information
HyperD is a registered trademark of Pall Corporation
Preterm infants are at risk of developing sepsis, necrotizing enterocolitis (NEC), chronic lung disease (CLD), and retinopathy of prematurity (ROP). We used high-throughput mass spectrometry to investigate whether cord blood proteins can be used to predict development of these morbidities.
The Journal of clinical endocrinology and metabolism, 94(8), 3038-3043 (2009-06-06)
GH is a known modulator of the immune system, but the effect of exogenous GH administration on white blood cell proteins has not been investigated. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) is a powerful platform for the study
The stationary phase sigma factor σ(S) (RpoS) controls a regulon required for general stress resistance of the closely related enterobacteria Salmonella and Escherichia coli. The σ(S)-dependent yncC gene encodes a putative DNA binding regulatory protein. Application of the surface-enhanced laser
Neurobiology of disease, 23(1), 61-76 (2006-03-22)
This paper describes the high-throughput proteomic analysis of the dorsolateral prefrontal cortex (DLPFC) from schizophrenia (SCHIZ), bipolar (BD), and normal control cohorts from the Harvard Brain Tissue Resource Center performed using ProteinChip technology based on the surface-enhanced laser desorption/ionization time
Methods in molecular biology (Clifton, N.J.), 357, 331-341 (2006-12-19)
Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (TOFMS) allows for rapid differential proteomic analysis of various experimental conditions. In the first step of retention chromatography, the proteome to be analyzed is fractionated based on the biochemical properties of the proteins or
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