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P9541

Sigma-Aldrich

Potassium chloride

for molecular biology, ≥99.0%

Synonym(s):

KCl

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About This Item

Linear Formula:
KCl
CAS Number:
Molecular Weight:
74.55
EC Number:
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.31
Assay:
≥99.0%
grade:
for molecular biology
form:
powder
Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

Assay

≥99.0%

form

powder

mp

770 °C (lit.)

density

1.98 g/mL at 25 °C (lit.)

cation traces

Fe: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

[Cl-].[K+]

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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General description

Potassium chloride (KCl) is useful in studies of ion transport and potassium channels.[1] KCl is known to be used in the crystallization of histone core octamers.[2]

Application

Potassium chloride has been used:
  • as a component of Danieau medium and also used in the preparation of phosphate buffer saline[3]
  • in the preparation of electrolyte solution for electrochemical impedance spectroscopy and cyclic voltammetry measurements[4]
  • as a component of HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)-buffered Tyrode′s solution[5]

May be used for the preparation of phosphate buffered saline, and for the extraction and solubilization of proteins.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Fluid osmolarity acutely and differentially modulates lymphatic vessels intrinsic contractions and lymph flow
Solari E, et al.
Frontiers in Physiology, 9(1), 871-871 (2018)
Reconstitution of nucleosome core particles from recombinant histones and DNA
Test, 375(20), 23-44 (2003)
Electrochemical impedance spectroscopic technique with a functionalized microwire sensor for rapid detection of foodbornepathogens
Lu L, et al.
Biosensors And Bioelectronics, 42(1), 492-495 (2013)
Diffusion through channel derivatives of the Escherichia coli FhuA transport protein
Braun M, et al.
European Journal of Biochemistry, 269(20), 4948-4959 (2002)
High-resolution in situ hybridization to whole-mount zebrafish embryos.
Thisse C and Thisse B
Nature Protocols, 3(1), 59-69 (2008)

Protocols

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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