MAK540
Beta-Hydroxybutyrate (Ketone Body) Assay Kit
sufficient for 200 colorimetric tests
Synonym(s):
Beta-Hydroxybutyrate Quantification Kit
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About This Item
UNSPSC Code:
41116104
NACRES:
NA.25
Recommended Products
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; gastrointestinal diseases; cancer
shipped in
dry ice
storage temp.
−20°C
General description
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop. The two main ketone bodies are beta-hydroxybutyrate (β-HB) and acetoacetate (AcAc), while acetone is the third abundant ketone body. Normally these two predominant ketone bodies are present in small amounts in the blood during fasting and prolonged exercise. In patients who have diabetes, alcohol or salicylate poisoning, hormone deficiency, childhood hypoglycemia and other acute disease states, large quantities of ketone bodies are found in the blood. The over-production and accumulation of ketone bodies in the blood (ketosis) can lead to pathological metabolic acidosis (ketoacidosis). Blood ketone testing methods that quantify beta-HB, the predominant ketone body in the blood (approximately 75%) have been used for diagnosing and monitoring treatment of ketoacidosis.
Suitability
The Beta-Hydroxybutyrate Assay Kit offers a sensitive colorimetric assay for measuring βHB levels in biological samples.
Principle
This assay is based on an enzyme coupled reaction of beta-HB, in which the product NADH can be specifically monitored by a NADH sensor. The signal can be measured by an absorbance microplate reader with the OD ratio at the wavelength of 570 nm to 610 nm. The detection limit of this assay kit is 4 µM beta-hydroxybutyrate in a 100 µL reaction volume.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 3
Certificates of Analysis (COA)
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