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L3038

Sigma-Aldrich

Lysenin from Eisenia foetida

solid

Synonym(s):

Eisenia Lysenin, Lysenin Protein

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About This Item

MDL number:
MFCD02687941
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

form

solid

Quality Level

200

mol wt

33 kDa

concentration

≥50% (SDS-PAGE)

storage temp.

−20°C

General description

Lysenin is a superfamily of certain proteins including lysenin-related protein 1 (LRP-1, lysenin 2) and LRP-2 (lysenin 3).

Application

Lysenin from Eisenia foetida has been used to treat B cells and study lysenin′s effect on membrane diacylglycerol (DAG) and surface sphingomyelin (SM).[1]

Biochem/physiol Actions

Lysenin is a 33kDa protein present in the coelomic fluid of the earthworm Eisenia foetida. It interacts with sphingomyelin in cell membranes. In vertebrates, this binding results in cytotoxicity and contraction of smooth muscle in vitro as well as vasodepressor activity and lethality under in vivo conditions.[2]
Lysenin serves as a tool to explore membrane lipid organization.[3] It is known to induce hemolysis in vertebrates and mammalian cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN3549
SLCL8440
SLCL1118
SLCK5598
SLCK0780

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Reiko Ishitsuka et al.
Anatomical science international, 79(4), 184-190 (2005-01-07)
Sphingomyelin is a major sphingolipid species in animal cells and is a major lipid constituent of plasma membranes. Recent reports have established important roles for sphingomyelin and its metabolites as second messengers in signal transduction events during development and differentiation.
Hideshi Kobayashi et al.
International review of cytology, 236, 45-99 (2004-07-21)
Lysenin is a protein of 33?kDa in the coelomic fluid (CF) of the earthworm Eisenia foetida. It differs from other biologically active proteins, such as fetidins, eiseniapore, and coelomic cytolytic factor (CCF-1), that have been found in Eisenia foetida, in
Peiqi Ou et al.
Cell reports, 36(9), 109624-109624 (2021-09-02)
B cell tolerance prevents autoimmunity by deleting or deactivating autoreactive B cells that otherwise may cause autoantibody-driven disorders, including systemic lupus erythematosus (lupus). Lupus is characterized by immunoglobulin Gs carrying a double-stranded (ds)-DNA autospecificity derived mainly from somatic hypermutation in
A Yamaji et al.
The Journal of biological chemistry, 273(9), 5300-5306 (1998-03-28)
Lysenin, a novel 41-kDa protein purified from coelomic fluid of the earthworm Eisenia foetida, induced erythrocyte lysis. Preincubation of lysenin with vesicles containing sphingomyelin inhibited lysenin-induced hemolysis completely, whereas vesicles containing phospholipids other than sphingomyelin showed no inhibitory activity, suggesting

Questions

  1. What's the solubility of the Lysenin samples in water? I desovate the 50ug sample in 0.6 mL water, looks a lot of not solutable? How to you usaually desovate the samples? In water or any buffers? I am woring on Lysenin reconstitution in to lipid bilyers.

    1 answer

    1. The solubility for the product is not analyzed on a lot to lot basis. However, it has been cited that solution were prepared by dissolving lysenin (1 mg/ml in 2% BSA-PBS) or 50 μg of lysenin in 50 μl of 10 mM HEPES/KOH (pH 7.0) containing 30 mM KCl and 50% Glycerol.

      Kindly review the following citations:
      Yamaji A, Sekizawa Y, Emoto K, Sakuraba H, Inoue K, Kobayashi H, Umeda M. Lysenin, a novel sphingomyelin-specific binding protein. J Biol Chem. 1998 Feb 27;273(9):5300-6. doi: 10.1074/jbc.273.9.5300. PMID: 9478988.

      Faouri RA, Krueger E, Govind Kumar V, Fologea D, Straub D, Alismail H, Alfaori Q, Kight A, Ray J, Henry R, Moradi M, Salamo G. An Effective Electric Dipole Model for Voltage-induced Gating Mechanism of Lysenin. Sci Rep. 2019 Aug 7;9(1):11440. doi: 10.1038/s41598-019-47725-0. PMID: 31391571; PMCID: PMC6686002.

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