I8135
IgM from bovine serum
reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution
Synonym(s):
Bovine IgM
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
conjugate
unconjugated
grade
reagent grade
Assay
≥95% (SDS-PAGE or HPLC)
form
buffered aqueous solution
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
General description
IgM antibodies are present as pentamers in the serum and are produced in response to antigens
IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.
IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells.
Application
IgM from bovine serum has been used for screening the lipopolysaccharide levels in Limulus amoebocyte lysate (LAL) assay. IgM has been used in flow cytometry assay for detecting FcγRIV expression and immunoglobulin binding.
Purified bovine IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.
Biochem/physiol Actions
IgM plays a key role in engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.
Physical form
Solution in 0.05 M Tris-HCl, 0.15 M sodium chloride and 0.01 M glycine, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
IgE b immune complexes activate macrophages through FcgammaRIV binding
Hirano M, et al.
Nature Immunology, 8(7), 762-762 (2007)
Lipopolysaccharide is a frequent and significant contaminant in microglia-activating factors
Weinstein JR, et al.
Glia, 56(1), 16-26 (2008)
Karen Yanson et al.
Journal of clinical microbiology, 59(12), e0101921-e0101921 (2021-09-30)
Nucleic acid amplification testing (NAAT) for SARS-CoV-2 is the standard approach for confirming COVID-19 cases. This study compared results between two emergency use authorization (EUA) NAATs, with two additional EUA NAATs utilized for discrepant testing. The limits of detection (LOD)
Gunasekaran Dhandapani et al.
Biotechnology and bioengineering, 119(7), 1997-2003 (2022-03-25)
Immunoglobulin M (IgM) antibodies hold promise as anticancer drugs and as agents for promoting immune homeostasis. This promise has not been realized due to low expression levels in mammalian cells producing IgM class antibodies, and the failure of protein A
Emerging functions of natural IgM and its Fc receptor FCMR in immune homeostasis
Wang H, et al.
Frontiers in Immunology, 7, 99-99 (2016)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service