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GERPN6302

Iodoacetamide

Cytiva RPN6302, pack of 25 g

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

form

solid

feature

wetted part: no

packaging

pack of 25 g

manufacturer/tradename

Cytiva RPN6302

color

Yellowish

solubility

cold water: soluble (Easily soluble in cold and hot water)

storage temp.

2-8°C

Related Categories

General description

Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.

Application

Iodoacetamide has been used:
  • as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
  • in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
  • as a protein alkylation agent

Biochem/physiol Actions

Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Features and Benefits

  • Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H301,H334,H317,H413

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

C5070

CHAPS hydrate

Iodoacetic acid ≥98.0% (T)

Sigma-Aldrich

I4386

Iodoacetic acid

Differential effects of iodoacetamide and iodoacetate on glycolysis and glutathione metabolism of cultured astrocytes
Schmidt M and Dringen R
Frontiers in Neuroenergetics, 1(13), 1-1 (2009)
COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal
Xu P, et al.
eLife, 6, e28342-e28342 (2017)
Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction
Roura-Martinez D, et al.
Journal of drug and alcohol research, 6(3), b1-b8 (2014)
Proteomic analysis of palmitoylated platelet proteins
Dowal L, et al.
Blood, 118(13), e62-e73 (2011)

Protocols

Rehydration Solutions

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service