G9293
Anti-GAL4, Activation domain antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Galactose 4
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5 μg/mL using GAL4-AD fusion protein in Saccharomyces cerevisiae extract
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
GAL4 protein is an 881 amino acid transcription factor containing an independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a regulatory gene that belongs to GAL gene family. It is a component of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).
Useful in the study of protein:protein interaction studies. GAL4 DNA activation domain fusion proteins.
Immunogen
Synthetic peptide corresponding to amino acids of Saccharomyces cerevisiae GAL4 protein.
Application
Anti-GAL4, Activation domain antibody produced in rabbit has been used in western blotting.
Biochem/physiol Actions
GAL4 induces the genes that are responsible for regulation of galactose metabolism in saccharomyces cerevisiae. GAL4 protein is widely used for studying transcriptional activation in eukaryotes. The yeast two hybrid system is widely used to study to discover protein− protein interactions (PPIs) and protein−DNA interactions.
Physical form
The product is provided as a solution of affinity isolated antibody in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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The putative SWI/SNF complex subunit BRAHMA activates flower homeotic genes in Arabidopsis thaliana.
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Arabidopsis thaliana BRAHMA (BRM, also called AtBRM) is a SNF2 family protein homolog of Brahma, the ATPase of the Drosophila SWI/SNF complex involved in chromatin remodeling during transcription. Here we show that, in contrast to its Drosophila counterpart, BRM is
Temperature-sensitive Mutations Made Easy Generating Conditional Mutations Using TS-inteins That Function Within Different Temperature Ranges
Tan G, et al.
Genetics, 62(1-2), 291-304 (2009)
Yeast Gal4: a transcriptional paradigm revisited
Traven A, et al.
EMBO Reports, 7(5), 496-499 (2006)
Alessandro Berto et al.
EMBO reports, 19(5) (2018-04-11)
Cenp-F is a multifaceted protein implicated in cancer and developmental pathologies. The Cenp-F C-terminal region contains overlapping binding sites for numerous proteins that contribute to its functions throughout the cell cycle. Here, we focus on the nuclear pore protein Nup133
Yeast two-hybrid, a powerful tool for systems biology
Bruckner A, et al.
International Journal of Molecular Sciences, 10(6), 2763-2788 (2009)
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