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G8255

Sigma-Aldrich

Glutamic-Pyruvic Transaminase from porcine heart

lyophilized powder, ≥75 units/mg protein

Synonym(s):

L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT

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About This Item

CAS Number:
9000-86-6
Enzyme Commission number:
2.6.1.2 (BRENDA, IUBMB)
Beilstein:
3562140
EC Number:
232-561-4
MDL number:
MFCD00131192
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Porcine heart

form

lyophilized powder

specific activity

≥75 units/mg protein

composition

Protein, 75-95%

technique(s)

cell culture | mammalian: suitable

color

light yellow to yellow

foreign activity

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.1%

storage temp.

−20°C

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General description

Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.

Research area: Cell Signaling

Application

Glutamic-Pyruvic Transaminase from porcine heart has been used:
  • in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
  • to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
  • in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies

Biochem/physiol Actions

Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physical form

Lyophilized powder containing citrate and acetate

Storage and Stability

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Analysis Note

Protein determined by biuret

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Giordano Pula et al.
Journal of biochemical and biophysical methods, 64(3), 167-181 (2005-08-23)
The surface expression of G protein-coupled receptors is regulated by internalization. For many receptors, a constitutive level of internalization in the absence of agonist has been reported. The constitutive internalization of metabotropic glutamate receptor 1a (mGluR1a) has been described, but
A Comprehensive Guide to Toxicology in Nonclinical Drug Development
Faqi A S
Biomarkers journal., 2017 null
A simple and sensitive detection of glutamic-pyruvic transaminase activity based on fluorescence quenching of bovine serum albumin
Chen M, et al.
Royal Society of Chemistry Advances, 5, 103557-103562 (2015)
Wei Guo et al.
Cancers, 12(7) (2020-07-15)
Metabolic reprogramming is an essential hallmark of cancer. Besides the "Warburg effect", cancer cells also actively reprogram amino acid metabolism to satisfy high nutritional demands in a nutrient-poor environment. In the glucose-alanine cycle, exogenous alanine taken up by hepatocytes is
Christoph Mahlert et al.
Journal of the American Chemical Society, 129(39), 12011-12018 (2007-09-06)
The acidic lipopeptides, including the calcium-dependent antibiotics (CDA), daptomycin, and A54145, are important macrocyclic peptide natural products produced by Streptomyces species. All three compounds contain a 3-methyl glutamate (3-MeGlu) as the penultimate C-terminal residue, which is important for bioactivity. Here
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