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D7304

Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride

Synonym(s):

Chromogenic substrate, DAB substrate solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

form

liquid

shipped in

wet ice

storage temp.

2-8°C

General description

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme and is useful as a staining agent in immunohistological and immunoblotting techniques. The end product is resistant to alcohol therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate System. The DAB Liquid Substrate System provides all the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution..

Application

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride has been used as a substrate:
  • in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver
  • for visualization of sections after immunostaining
  • in immunohistochemistry of mice knee joint sections
  • in the immunolabeling of kisspeptin neurons in the preoptic area of camel
It produces a brown precipitate with peroxidase for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures.

Caution

Stable at least one year at 2-8 °C.

Quantity

Each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kit Components Only

Product No.
Description
  • 10X DAB Liquid Chromogen 25 mL
  • ready-to-use buffer/peroxide solution 250 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Certificates of Analysis (COA)

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S R Pitkin et al.
Behavioural brain research, 119(2), 167-177 (2001-02-13)
The present study aimed to assess the role of advanced age in the development and manifestation of thiamine deficiency using an animal model of Wernicke-Korsakoff syndrome (WKS). Interactions between pyrithiamine-induced thiamine deficiency (PTD) and age were examined relative to working
K M Mader et al.
Genes, brain, and behavior, 7(7), 740-753 (2008-06-04)
Mucopolysaccharidosis type IIIA (MPS IIIA) is an inherited neurodegenerative lysosomal storage disorder characterised by progressive loss of learned skills, sleep disturbance and behavioural problems. Reduced activity of sulphamidase (SGSH; EC 3.10.1.1) results in intracellular accumulation of heparan sulphate (HS), with
The dromedary camel displays annual variation in hypothalamic kisspeptin and Arg-Phe-amide-related peptide-3 according to sex, season, and breeding activity
Ainani H, et al.
The Journal of Comparative Neurology, 528(1), 36-51 (2020)
J Balthazart et al.
Neuroscience, 153(4), 944-962 (2008-05-02)
Songbirds produce learned vocalizations that are controlled by a specialized network of neural structures, the song control system. Several nuclei in this song control system demonstrate a marked degree of adult seasonal plasticity. Nucleus volume varies seasonally based on changes
Christian Dölle et al.
Scientific reports, 8(1), 1272-1272 (2018-01-21)
3,3'-Diaminobenzidine (DAB) is a widely used chromogen in histological staining methods and stained tissue is often used in downstream molecular analyses such as quantitative PCR (qPCR). Using microdissected muscle fibers from sequential muscle sections stained by DAB-dependent and -independent methods
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