SSE-5

95122021, fish embryo, Epithelial-like

• UNSPSC Code: 41106514

Properties

• Product Name: SSE-5, 95122021
• biological source: fish embryo
• growth mode: Adherent
• karyotype: Modal no. 55
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Fish Sockeye Salmon embryo

Cell Line Description

SSE-5 was initiated in 1964 from pooled embryos of Sockeye Salmon (Oncorynchus nerka). The cell line that is also known as Sockeye Salmon embryo-5, is susceptible to infectious pancreatic necrosis virus (IPNV), viral hemorrhagic septicaemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). For replication of salmonid viruses SSE-5 cells should be grown at 18°C.

Application

Isolation of viruses from clinical samples, virus propagation

Culture Medium

MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at leas

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.