BF-11
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
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biological source
Rat x Mouse Hybridoma
packaging
tube of 5 μg 95050413-DNA-5UG
pkg of vial of cells 95050413-1VL
ImmunoDon
Rat AC1 spleen cells
Myeloma
Sp2/0 Ag-14
Reactivity
Anti-Erythropoietin, human
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat ACI spleen x Mouse Sp2/0 Ag-14
Cell Line Description
The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.
Immunogen
Human erythropoietin
Application
Immunological studies, isolation of erythropoietin by immuno-affinity methods.
The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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