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Key Documents

C3090

Sigma-Aldrich

pcDNA 1.1 (−)/Amp/CAT control vector

lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
12352200

form

lyophilized powder

storage temp.

−20°C

Specificity

The MCS contains unique BamH I, EcoR I, EcoR V, Hind III, Not I, Nsi I, Sph I, Xba I, and Xho I restriction sites, and redundant BstX I sites.

Features and Benefits

A 4.8-kb vector for transient, high-level protein expression in mammalian cells. The vector incorporates the strong cytomegalovirus (CMV) enhancer-promoter sequence for high-level expression, SV40 and polyoma origins of replication to facilitate episomal replication, an ampicillin resistance gene for selection in E. coli, and the col E1 origin and supF suppressor tRNA for growth and maintenance in appropriate strains of E. coli.

Other Notes

This is the pcDNA1.1/Amp vector with an insert containing the chloramphenicol acetyltransferase gene.

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Certificates of Analysis (COA)

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