C-12575

Human Uterine Smooth Muscle Cells (HUtSMC)

500,000 cryopreserved cells

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: human uterus (myometrium)
• packaging: pkg of 500,000 cells
• morphology: (smooth muscle)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Cell Line Origin

Uterus

Application

Primary Human Uterine Smooth Muscle Cells (HUtSMC) are isolated from the myometrium (the middle layer of the uterine wall) and provided in a cryopreserved format. HUtSMC stain positive for smooth muscle α-actin. During pregnancy, hormones and growth factors cause an extensive hypertrophy of uterine smooth muscle cells. This and other morphological and biochemical effects on the uterus can be studied in vitro using HUtSMC.

Quality

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

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Other Notes

Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable