C0249

Anti-CSTF3 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CSTF-77, Anti-Cleavage stimulation factor 77
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~77 kDa
• species reactivity: human
• technique(s): immunoprecipitation (IP): 2.5-5 μL using lysates of HEK-293T cells; western blot: 1:500-1:1000 using lysates of HEK-293T
• UniProt accession no.: Q12996
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CSTF3(1479)

Description

General description

Cleavage stimulatory factor (CstF3), also called CSTF-77, is a component of CstF heterotrimer. It has a proline-rich domain and the gene is mapped to human chromosome 11p13. CstF3 contains 12 half-A-TPR (HAT) repeats followed by a proline-rich segment.

Specificity

Anti-CSTF3 (N-terminal) specifically recognizes human CSTF3 (also known as Cleavage stimulation factor 77, CSTF-77, CF-1 77 kDa subunit).

Application

Anti-CSTF3 (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Cleavage stimulatory factor (CstF3) is required for proper 3′-end cleavage and is critical for the assembly of the CstF complex, bridging both CSTF1 and CSTF2. CSTF3 also interacts directly with the RNA polymerase II C-terminal domain (CTD), but with lower efficiency than CSTF1.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable