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A6154

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Rabbit IgG (whole molecule)–HRP

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About This Item

MDL number:
MFCD00162788
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is immunospecific for rabbit IgG by immunoelectrophoresis against normal serum and rabbit IgG, prior to conjugation.

Immunogen

purified rabbit IgG

Application

C33A cell lysates were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Protein aliquots from colonic mucosal extracts (μg) were anlayzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:5000 dilution in TBSt for 1 hr at room temperature.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody has been used for western blot (1:6,000), and ELISA assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Other Notes

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Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Johan de Vogel et al.
Carcinogenesis, 29(2), 398-403 (2008-01-05)
Epidemiological and animal model studies suggest that a high intake of heme, present in red meat, is associated with an increased risk of colon cancer. The aim of this study was to elucidate the effects of dietary heme on colonic
Pascal Nurwakagari et al.
Journal of molecular endocrinology, 38(1-2), 259-275 (2007-02-13)
Glycoprotein hormone receptors such as the lutropin/chorionic gonadotropin receptor (LHR) are characterized by a large N-terminal ectodomain (ECD), which is responsible for hormone-receptor interactions. For the closely related TSH receptor (TSHR), it has been proposed that the ECD also serves
A Moles et al.
Oncogene, 35(35), 4623-4632 (2016-02-09)
Post-translational modifications of nuclear factor (NF)-κB subunits provide a mechanism to differentially regulate their activity in response to the many stimuli that induce this pathway. However, the physiological significance of these modifications is largely unknown, and it remains unclear if
Steffen Erkelenz et al.
Retrovirology, 12, 29-29 (2015-04-19)
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Journal of virology, 85(1), 178-188 (2010-10-29)
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