This product has not been specifically tested for use in antioxidant assays. However, it has been used successfully as such in several publications. Please see the link below to review one example:
https://www.sciencedirect.com/science/article/pii/S0009912003002315?via%3Dihub
A3219
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
peroxidase substrate, chromogenic, liquid
Synonym(s):
ABTS
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Product Name
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Liquid Substrate System
Quality Level
form
liquid
storage temp.
2-8°C
SMILES string
CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O
InChI
1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-
InChI key
ZTOJFFHGPLIVKC-CLFAGFIQSA-N
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General description
The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.
Application
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product.[1][2] It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.[3]
Biochem/physiol Actions
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically.[4] A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples.[5] ABTS method is flexible at varying pH and most widely used.[6] ABTS is a substrate for phenol oxidase enzyme.[7]
Physical form
Ready-to-use.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Can this product be used for antioxidant assay?
1 answer-
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What is the approximate concentration of H2O2 in the ready-to-use A3219 solution?
1 answer-
This product is no longer tested for hydrogen peroxide concentration. The previous specification for H2O2 was </= 0.03%. Current lots are expected to be within this range.
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What is the molecular weight and density?
1 answer-
This product is a 1.8 mM solution of ABTS, the density is not determined. The solution is prepared from a diammonium salt form with a molecular weight of 548.60.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/745/a3219dat.pdfHelpful?
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ABTS ready to use를 사용하여 항산화 실험을 하는경우 기존의 방법에 적용하면 어떤 단계가 생략이 되는건지 자세한 실험 과정을 알고 싶습니다
1 answer-
The only protocol currently available on the website is this data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/745/a3219dat.pdf.
It may be helpful to also view the "Peer-Reviewed Papers" related to this product: https://www.sigmaaldrich.com/search/a3219?focus=papers&page=1&perpage=30&sort=relevance&term=a3219&type=citation_search.
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Should Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), be diluted prior to use?
1 answer-
No, this product is offered as a ready-to-use (1×) solution.
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What is the concentration of Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)?
1 answer-
The concentration of the ABTS is approximately 1.8 mM.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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When using Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), at what wavelength should the product of the reaction be measured?
1 answer-
The product should be measured at 405 nm. For end point assays, the reaction may be stopped with 1% (w/v) SDS and also read at 405 nm.
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