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45260

Sigma-Aldrich

Eosin B

for microscopy (Fl., Hist.)

Synonym(s):

4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine

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About This Item

Linear Formula:
C20H6N2O9Br2Na2
CAS Number:
548-24-3
Molecular Weight:
624.06
Colour Index Number:
45400
Beilstein:
4121964
EC Number:
208-943-1
MDL number:
MFCD00005041
UNSPSC Code:
12171500
PubChem Substance ID:
329756626
NACRES:
NA.47
Pricing and availability is not currently available.

grade

for microscopy (Fl., Hist.)

technique(s)

titration: suitable

λmax

509-524 nm

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

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General description

Eosin B is a fluorescent red dye that is used in microscopy to visualise cytoplasm and proteins like collagen.[1]

Application

Eosin B is an adsorption and fluorescent indicator in microscopy.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK9334
BCCD7122
BCCD0415
BCCC9309
BCCC1582

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Rebecca Pastrana-Mena et al.
The Journal of biological chemistry, 285(35), 27045-27056 (2010-06-25)
Malaria parasites contain a complete glutathione (GSH) redox system, and several enzymes of this system are considered potential targets for antimalarial drugs. Through generation of a gamma-glutamylcysteine synthetase (gamma-GCS)-null mutant of the rodent parasite Plasmodium berghei, we previously showed that
A A Waheed et al.
Journal of biochemical and biophysical methods, 42(3), 125-132 (2000-03-29)
Eosin B is used to estimate proteins above 1 microg/ml concentration [Waheed AA, Gupta, PD. Anal. Biochem. 1996:233:249-256; Waheed AA, Gupta PD. J. Biochem. Biophys. Meth. 1996;33:187-196]. In the present report we describe a method for estimating submicrogram quantities of
Zhong-Xin Zhu et al.
Analytical biochemistry, 426(1), 1-3 (2012-04-10)
A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, stained, and developed within 30 min to achieve transparent and colorless LPS
Y M Cheng et al.
Neuroscience, 151(4), 1084-1098 (2008-01-19)
We examined the potential contribution of a voltage-gated proton conductance (gH+) to acid extrusion from cultured postnatal rat hippocampal neurons. In neurons loaded with Ca2+- and/or pH-sensitive fluorophores, transient exposures to 25-139.5 mM external K+ (K+o) or 20 microM veratridine
A A Waheed et al.
Journal of biochemical and biophysical methods, 33(3), 187-196 (1996-12-30)
We describe a detailed procedure for estimating a wide range of proteins by the eosin B dye method. At acidic pH, eosin B binds to proteins and absorption of the protein-dye complex at 536-544 nm is proportional to the concentration
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