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VirusExpress® 293 AAV Production Cells

Pharma Manufacturing

Synonym(s):

HEK293 cells for AAV production

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About This Item

Quality Level

600

shipped in

liquid nitrogen

storage temp.

−196°C

General description

VirusExpress® 293 AAV Production Cells are a clonal, suspension-adapted HEK293 derivative selected for fast growth, high transfection efficiency, and high-titer AAV production. VirusExpress 293 AAV Production Cells grow to high cell densities and are amenable to chemical-based transfection reagents commonly used in manufacturing (i.e., PEI, lipid-nanoparticle). A medium exchange step is not needed prior to transfection, and the cells can be transfected in larger volumes, enabling scale-up of AAV production. The cells are supplied in a chemically defined media formulation and can be thawed directly into EX-CELL® CD HEK293 Viral Vector Medium.

VirusExpress 293 AAV Production Cells are derived from the HEK293 cell line (ATCC CRL-1573) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham F.L. et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.

Packaging

This product is shipped in a liquid nitrogen dry vapor shipper. The liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt.

Other Notes

The cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL. The cells were banked under cGMP conditions in chemically defined medium and 8% DMSO.

Legal Information

A commercial license must be taken prior to use of the cells, or any products or materials derived from the cells, in a manufacturing process. Please contact your local sales representative for more details.
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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