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70137

Millipore

ENDO Agar (Base)

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
di-potassium hydrogen phosphate, 3.5 g/L
lactose, 10 g/L
peptone, 10 g/L
sodium sulfite, 2.5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.5±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Enterobacter spp.
selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for Proteus spp.
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for coliforms

Application

A selective medium for the differentiation of lactose fermenting and lactose non-fermenting intestinal organisms.

Preparation Note

Suspend 41 g in 1 litre of distilled water. Add 4 ml of 10% w/v alcoholic solution (96% ethyl alcohol) of basic fuchsin (Cat. No. 47860). Bring to boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Mix well before pouring.

Other Notes

The assessment of ammonia-induced cell envelope injury in E. coli and Enterobacter aerogenes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF4745
BCCD5839
BCBW0309
BCBR2270V
BCBR2269V

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G Naundorf et al.
Canadian journal of microbiology, 36(8), 525-529 (1990-08-01)
Ammonia-induced cell envelope injury was examined in pure cultures of Escherichia coli and Enterobacter aerogenes. Cell injury, as determined by the ratio of colony-forming units on m-T7 agar to colony-forming units on m-Endo agar, increased with exposure to increasing concentrations
S. Endo
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. 1. Abt. Medizinisch-Hygienische Bakteriologie, Virusforschung und Parasitologie. Originale, 35, 109-109 (1904)

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