11873628001

Pefabloc^® SC PLUS

solution, suitable for electrophoresis, solubility: 0.1 M in water, soluble, pkg of 2 × 25 mL (1 g)

• Synonym(s): PSC-protector, serine protease inhibitor
• UNSPSC Code: 12352204

Properties

• form: solution
• mol wt: 239.5
• packaging: pkg of 2 × 25 mL (1 g)
• manufacturer/tradename: Roche
• concentration: 0.1-1.0 mg/mL
• technique(s): electrophoresis: suitable
• mp: 175-185 °C
• solubility: water: soluble 0.1 M
• storage temp.: 2-8°C

Description

General description

Set consists of Pefabloc^® SC and the corresponding amount of PLUS additive to avoid covalent attachment of Pefabloc^® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. Choose Pefabloc^® SC for all applications where the general inhibition of serine proteases is desired. With its high stability and irreversible inhibition mechanism, Pefabloc^® SC protects proteins throughout procedures such as:

• Extraction processes (from animal tissues or cells, plants, bacteria, yeast, and fungi)
• Subsequent purification steps
• Varied sample storage conditions
• Downstream protein detection (e.g., western blotting, reporter gene analysis, etc.)
• Biochemical studies where proteins are required.

Use Pefabloc^® SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc^® SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis.

The Pefabloc^® SC PLUS set combines the serine protease inhibitor Pefabloc^® SC with a uniquely formulated Pefabloc^® SC protector (PSC-Protector), adding additional convenience and reliability to the safe, stable protease inhibition provided by Pefabloc^® SC .

Recent findings indicate that sulfonyl-type serine protease inhibitors such as Pefabloc^® SC and PMSF can bind covalently to proteins. This can occur when the inhibitors are used in high concentrations, or during extended incubation times under alkaline conditions. This interaction adversely affects the tyrosine and lysine residues of a protein, as well as the free amino terminus. The PLUS additive (PSC-Protector) included with this set prevents covalent attachment of Pefabloc^® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH.

Specificity

Specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity of Pefabloc^® SC is comparable to PMSF or DFP; however, Pefabloc^® SC is non-toxic. Specifically inhibits serine proteases and prevents nonspecific covalent modification of proteins as tested by mass spectrometry.

Features and Benefits

• Take advantage of a simplified two-reagent system. Use the PSC-Protector solution to prevent covalent binding between proteins and Pefabloc^® SC, even at high concentrations, extended incubation times, and at alkaline pH (see Figure 1 and Figure 2).
• Benefit from an easy-to-use inhibitor. Add water-soluble Pefabloc^® SC directly to aqueous buffers.
• Avoid hazardous compounds. Obtain non-toxic protease inhibition without risk to you, or those around you.
• Ensure protection with improved stability. Achieve consistent protease inhibition even at pH levels above 7.0 and temperatures above +4°C.
• Maximize inhibition. Be certain that your levels of active inhibitor are high enough by using a product that is reliably soluble and stable.

Packaging

Set consists of Pefabloc^® SC and the corresponding amount of PLUS additive to avoid covalent attachment of Pefabloc^® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. Choose Pefabloc^® SC for all applications where the general inhibition of serine proteases is desired. With its high stability and irreversible inhibition mechanism, Pefabloc^® SC protects proteins throughout procedures such as:

• Extraction processes (from animal tissues or cells, plants, bacteria, yeast, and fungi)
• Subsequent purification steps
• Varied sample storage conditions
• Downstream protein detection (e.g., western blotting, reporter gene analysis, etc.)
• Biochemical studies where proteins are required.

Use Pefabloc^® SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc^® SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis.

Set containing two components

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Pefabloc is a registered trademark of Pentapharm

Kit Components Only

• Pefabloc SC, 1 g
• PSC-Protector solution, 2 x 25 mL ready-to-use

Safety Information

• Pictograms: GHS02,GHS05
• Signal Word: Danger
• Hazard Statements: H226,H314
• Precautionary Statements: P210 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378
• Hazard Classifications: Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1B
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1