MAB8131
Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2
clone 6F8.2, Chemicon®, from mouse
Synonym(s):
CMV
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F8.2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2a
shipped in
wet ice
Specificity
Reacts with an immediate early protein M.W. 68-72 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and/or intranuclear inclusion staining which reaches peak intensity between 8-24 hours.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
Immunogen
Affinity purified early antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Epitope: immediate early
Application
Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2 is an antibody against Cytomegalovirus for use in IF, WB, IC, IH(P).
Immunohistochemistry.
Immunocytochemistry.
Western Blot 1:100
IFA at 1:600-1:1,200 on acetone fixed cells.
Works on paraffin embedded tissue sections.
Final working dilutions must be determined by end user.
Immunocytochemistry.
Western Blot 1:100
IFA at 1:600-1:1,200 on acetone fixed cells.
Works on paraffin embedded tissue sections.
Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Linkage
Replaces: MAB8130
Physical form
Format: Purified
Purified immunoglobulin. Supplied in PBS buffer, pH 7.4. Contains 0.1% sodium azide and carrier protein. Has been sterile filtered.
Storage and Stability
Maintain at +4°C for up to 12 months
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Dominique McCormick et al.
mBio, 9(3) (2018-06-28)
As obligate intracellular parasites, viruses are completely dependent on host factors for replication. Assembly and egress of complex virus particles, such as human cytomegalovirus (HCMV), are likely to require many host factors. Despite this, relatively few have been identified and
Extensive human cytomegalovirus (HCMV) genomic DNA in the renal tubular epithelium early after renal transplantation: Relationship with HCMV DNAemia and long-term graft function.
Li YT, Emery VC, Surah S, Jarmulowicz M, Sweny P, Kidd IM, Griffiths PD, Clark DA
Journal of Medical Virology null
Yao-Tang Lin et al.
PLoS pathogens, 16(9), e1008844-e1008844 (2020-09-05)
The genomes of RNA and small DNA viruses of vertebrates display significant suppression of CpG dinucleotide frequencies. Artificially increasing dinucleotide frequencies results in substantial attenuation of virus replication, suggesting that these compositional changes may facilitate recognition of non-self RNA sequences.
Yao-Tang Lin et al.
PLoS pathogens, 13(5), e1006329-e1006329 (2017-05-12)
The human cytomegalovirus major immediate early proteins IE1 and IE2 are critical drivers of virus replication and are considered pivotal in determining the balance between productive and latent infection. IE1 and IE2 are derived from the same primary transcript by
Mapping the viral genetic determinants of endothelial cell tropism in human cytomegalovirus.
Bolovan-Fritts, Cynthia A and Wiedeman, Jean A
Journal of Clinical Virology, 25 Suppl 2, S97-109 (2002)
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