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14-453-M

Sigma-Aldrich

Akt1/PKBα (δPH, S473D) Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant human Akt1, residues 118-end containing the mutation S473D, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 45 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

UniProt accession no.

General description

N-terminal His6-tagged, recombinant human Akt1, residues 118-end containing the mutation S473D
Product Source: Human Akt1 expressed by baculovirus in Sf21 insect cells.

Biochem/physiol Actions

Protein Target: Akt1/PKBα
Target Sub-Family: AGC

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-453M when ordering the 250 µg size.

Quality

Routinely evaluated by phosphorylation of Crosstide substrate peptide

Physical form

Ni2+/NTA-agarose

Storage and Stability

1 year at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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