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1.05567

Supelco

TLC aluminum sheets, Silica gel 60/Kieselguhr F₂₅₄

pkg of 25 sheets, sheet L × W 20 cm × 20 cm, aluminum support

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About This Item

UNSPSC Code:
41115711
NACRES:
NB.21

material

aluminum support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 25 sheets

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

sheet L × W

20 cm × 20 cm

particle size

10-12 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

25 Aluminium sheets 20 x 20 cm
Precoated Kieselguhr or mixed Silica gel/Kieselguhr plates are only of small importance nowadays. Kieselguhr layers are used like cellulose layers for the TLC separation of polar and moderately polar substances. Polyamide, on the other hand , is used for the separation of flavonoids and other phenolic substances.

Linkage

Replaces: 5567-7; 5567

Analysis Note

Layer thickness: 170 - 220 µm
Deviation of layer thickness per plate: ≤ 30 µm
Chromatografic testings:
A) colour test
hRf-values
- bleu vif organol, colour test, lipophile:
20 - 30
- Ceres Black G, colour test, lipophile: 50 - 60
- ceres Violet brn, colour test, lipophile: 60 - 70
Typical value determined on a conditioned sheet
Eluent: toluene (45% rel. humidity)
B) PHB- Ester
- phb-methylester, phb-ester-test: 20 - 30
- phb-ethylester, phb-ester-test: 30 - 40
- phb-propylester, phb-ester-test: 40 - 50
Typical value determined on a sheet
Eluent: Pentane/ glacial acetic acid (88/12 v/v)

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