1.00188

Chromocult^® RAMBACH^™ Agar

ISO 6579, Specialized media for the isolation and differentiation of Salmonella spp. from food and animal feed, water and other materials.

• Synonym(s): Chromogenic Salmonella Agar, Propylene Glycol Deoxycholate Neutral Red Agar, Salmonella Agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: ISO 6579; suitable for ISO
• Quality Level: 200
• form: powder
• technique(s): microbiological culture: suitable
• pH: 7.3±0.2 (25 °C)
• application(s): food and beverages
• storage temp.: 15-25°C
• suitability: selective for enterobacteriaceae: Salmonella spp.

Description

General description

Chromocult^® RAMBACH^™ Agar is specifically designed for the differentiation of Salmonella species. The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.

Application

The Chromocult^® RAMBACH^™ Agar can be used for: -

• The detection of Salmonella in foodstuff, water, animal feed
• The differential diagnosis of Salmonella species in various samples

Analysis Note

• The dehydrated medium appears as dark-green color.
• The prepared medium is opaque and light pink in appearance.
• The pH value at 25 degrees is in the range of 7.1-7.5.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Rambach is a trademark of Balel S.A.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3