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07-1431

Sigma-Aldrich

Anti-Tie-2 Antibody, NT

from rabbit, purified by affinity chromatography

Synonym(s):

CD202b antigen, TEK tyrosine kinase, endothelial, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multip

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, rat

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TEK(7010)

General description

Tie-2 is a member of the tyrosine protein kinase family, is a transmembrane receptor for angiopoietin 1, and may represent one of the earliest markers for cell lineage within mammalian endothelial cells. Its expression is almost only found in human, mouse and rat endothelial cells and angioblasts. Most likely, Tie-2 regulates the differentiation, proper dispersal, and proliferation of these cells during angiogenesis. Mutations in TEK are a cause of VMCM or dominantly inherited venous malformations which are characterized by serpiginious, dilated veins. Tie-2 can be found in placenta and lung tissue. Lower levels of the protein can also be found in kidney, umbilical vein endothelial cells, and brain.

Specificity

Recognizes human Tunica internal endothelial cell kinase (TIE-2), also described as Angiopoietin-1 receptor, p140 TEK, or tyrosine protein kinase receptor. The peptide sequence used as immunogen is 100% conserved in human, rat, mouse, and 94% in bovine.

Immunogen

18 amino acid peptide sequence within the extracellular, N-terminus of human Tie-2

Application

Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
This Anti-Tie-2 Antibody, N-terminus is validated for use in WB for the detection of Tie-2.

Quality

Evaluated by Western Blot in human brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected Tie-2 in 10 µg of human brain lysate.

Target description

Approx. 126 kDa

Linkage

Replaces: AB3126

Physical form

Affinity purified
Affinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5, containing 0.2% BSA and 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Human brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Comparatine analysis of cytophenotypes of cells of mesenchymal lineage isolated from human tissues.
Yu G Suzdal'tseva,V V Burunova,N V Petrakova,I V Vakhrushev,K N Yarygin,V N Yarygin
Bulletin of experimental biology and medicine null

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