Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others
Application
Anti-Nitrotyrosine Antibody, clone 1A6 detects level of Nitrotyrosine & has been published & validated for use in IC, IH, IP & WB.
Quality
routinely evaluated on peroxynitrite-treated A431 cells; no reaction was noted with non-nitrated proteins
Target description
Predicted MW for nitrated-BSA: 66 kDa
Physical form
Format: Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.
Analysis Note
Control Immunohistochemistry: Human Alzheimer disease brain sections (Methacarn fixed), mouse liver Immunoblotting: Nitrated-BSA Indirect ELISA: nitrated-BSA Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Journal of Pharmacology and Experimental Therapeutics null
Relaxin ameliorates hypertension and increases nitric oxide metabolite excretion in angiotensin II but not N(?)-nitro-L-arginine methyl ester hypertensive rats.
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