X4626

XTT sodium salt

powder, BioReagent, suitable for cell culture

• Synonym(s): 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
• Empirical Formula (Hill Notation): C₂₂H₁₆N₇NaO₁₃S₂
• CAS Number: 111072-31-2
• Molecular Weight: 673.52
• NACRES: NA.75
• PubChem Substance ID: 24902150
• UNSPSC Code: 12352207
• MDL number: MFCD00083517

Properties

• product line: BioReagent
• Quality Segment: 200
• assay: ≥90%
• form: powder
• storage condition: dry at room temperature
• technique(s): cell culture | mammalian: suitable, protein quantification: suitable
• application(s): detection
• detection method: colorimetric
• storage temp.: 2-8°C
• SMILES string: [Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O
• InChI: 1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1
• InChI key: JACYMBNQPPWQML-UHFFFAOYSA-M

Description

General description

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Application

XTT sodium salt has been used to assess cell viability.

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

Biochem/physiol Actions

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

Safety Information

• pictograms: GHS02
• signalword: Danger
• hcodes: H242
• pcodes: P210 - P234 - P235 - P240 - P370 + P378 - P403
• Hazard Classifications: Self-react. C
• supp_hazards: EUH044
• Storage Class: 4.1A - Other explosive hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)