The 'type' is a historical designation used to differentiate the various Urease products and generally indicates a specific method of isolation and/or purification. The Type IX undergoes dialysis against deionized water prior to lyophilization. The process is EDTA-free, but may leave traces of potassium phosphate buffer salts. This item has an activity range of 50000 - 100000 units per gram solid. The mass of the 20KU option may vary from 200 mg – 400 mg per vial. The lot specific activity is published in the Certificate of Analysis.
U4002
Urease from Canavalia ensiformis (Jack bean)
Type IX, powder, 50,000-100,000 units/g solid
Synonym(s):
Jack bean urease, Urea amidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
Specific activity:
50,000-100,000 units/g solid
Pricing and availability is not currently available.
type
Type IX
Quality Level
form
powder
specific activity
50,000-100,000 units/g solid
mol wt
~544620 Da
storage temp.
2-8°C
General description
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.
Application
Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.[1]
Biochem/physiol Actions
In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline. [2]
Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.
Other Notes
One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 50,000-100,000 units/g solid | specific activity ≥600,000 units/g solid | specific activity ~1 U/mg | specific activity - |
| form powder | form powder | form powder | form glycerol solution |
| mol wt ~544620 Da | mol wt ~544620 Da | mol wt Mr ~480000 | mol wt ~544620 |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| type Type IX | type Type C-3 | type - | type Type III |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
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Related Content
An investigation of the use of urease-antibody conjugates in enzyme immunoassays
Chandler HM, et al.
Journal of Immunological Methods, 53(2), 187-194 (1982)
Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform
Kunduru KR, et al.
Test, 590, 143-167 (2017)
G A Rosenthal et al.
Plant physiology, 76(2), 541-544 (1984-10-01)
l-Canavanine, the guanidinooxy structural analog of l-arginine, is an important nonprotein amino acid of many leguminous plants with nitrogen storage a major proported role. l-[Guanidinooxy-(14)C]canavanine, [(14)C] urea, and [(15)N]urea were injected separately into the fleshy, green cotyledons of 9-day old
Global Trade Item Number
| SKU | GTIN |
|---|---|
| U4002-1MU | 04061832759982 |
| U4002-500KU | 04061832760001 |
| U4002-20KU | 04061837409523 |
| U4002-100KU | 04061837409516 |
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Hi there I was wondering what Type IX means and how many grams are there in the 20 kU one?
1 answer-
Helpful?
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Good morning, I would like to know what the difference is between the different types of urease, since some are type III, type IX, type C-III.
1 answer-
The type designations for urease products are not indications of particular isoforms. They are internal codes that refer to the chronological order of the products' market entry and manufacturing processes. The manufacturing processes are proprietary. However, the major differences between the urease products are their specifications for specific activity. Please access the 'Specification Sheet' and 'Sample Certificate of Analysis' for each individual product via its corresponding website.
Helpful?
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Hi there I was wondering what Type IX means and how many grams are there in the 20 kU one?
1 answer-
The 'type' is a historical designation used to differentiate various Urease products. Each lot has a specific activity of 50,000 - 100,000 units per gram solid. Therefore, the mass of the 20 kU option can vary between 0.2 g and 0.4 g. The lot-specific certificate of analysis will provide the specific activity.
Helpful?
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