The reconstituted urease solution is stable for at least one month when stored at 2–8 °C. Stability may be further extended by freezing. The stability is not effected following multiple freeze–thaw cycles. When reconstituted in 30 mL water, the solution will contain 100 units urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
U3383
Urease Buffer Reagent
Synonym(s):
Urea colorimetric determination assay reagent
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Pricing and availability is not currently available.
description
When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
Quality Segment
form
powder
mol wt
545 kDa
storage temp.
2-8°C
General description
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants. Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections. two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.
Application
Urease Buffer Reagent has been used:
- to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method
- in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells
- to study the metabolic capacity of rat hepatocytes
Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.
1 of 1
This Item | |||
|---|---|---|---|
| description - | description powder, ~1 U/mg | description Type III, powder, 15,000-50,000 units/g solid | description powder, white, ~8 U/mg |
| Quality Level 200 | Quality Level 100 | Quality Level 300 | Quality Level 100 |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt 545 kDa | mol wt Mr ~480000 | mol wt ~544620 Da | mol wt Mr ~480000 |
| description When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative. | description - | description - | description - |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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How long can we keep the solution after reconstitution? What is the recommended storage condition after reconstitution?
1 answer-
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What's the concentration of Jack bean Urease? I can only see the concentration of buffer.
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As provided, this product contains 100 units per vial. The mass weight per vial is not determined.
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How many urease units does 1 vial contain?
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As provided, this product contains 100 units per vial.
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What is the volume of water used for reconstitution of 1 vial?
1 answer-
As mentioned on the sample Certificate of Analysis, 30 mL of purified water in one vial of this material yields a clear solution.
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