T9378

Trypsin inhibitor

crude powder

• Synonym(s): Trypsin Inhibitor from lima beans
• CAS Number: 9035-81-8
• UNSPSC Code: 12352202
• NACRES: NA.77
• EC Number: 232-906-9
• MDL number: MFCD00082102

Properties

• Product Name: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• biological source: Phaseolus limensis (lima bean)
• Quality Segment: 200
• type: Type II-L
• form: crude powder
• mol wt: 9 kDa
• solubility: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• storage temp.: 2-8°C

Description

General description

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Biochem/physiol Actions

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Preparation Note

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Analysis Note

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

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Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)