In general, 'short term storage' refers to the material being stable for a period of days to weeks. In order to assure optimum performance, the end user may want to discontinue use of solutions stored refrigerated for 1 week or more as the loss of activity over time has not been investigated.
T9003
Trypsin inhibitor
lyophilized powder
Synonym(s):
SBTI
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About This Item
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Product Name
Trypsin inhibitor from Glycine max (soybean), lyophilized powder
biological source
Glycine max (soybean)
Quality Segment
form
lyophilized powder
mol wt
20.1 kDa
solubility
H2O: >10 mg/mL, phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), soluble
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Application
Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin. [1]
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Analysis Note
One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein.
Other Notes
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
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This Item | |||
|---|---|---|---|
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| Quality Level 300 | Quality Level 300 | Quality Level 400 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility H2O: >10 mg/mL, soluble, phosphate buffer: >10 mg/mL (hazy above 10 mg/ml) | solubility balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble | solubility 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL, clear, colorless to very faintly yellow | solubility balanced salt solution: 1 mg/mL, serum-free medium: soluble |
| mol wt 20.1 kDa | mol wt 20,100 Da | mol wt 20 kDa | mol wt 20.1 kDa |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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what is the time period for "short term storage" at 4C after reconstitution of the powder?
1 answer-
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
1 answer-
Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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