T4532

Tryptose Broth

suitable for insect cell culture

• NACRES: NA.75
• UNSPSC Code: 41106507

Properties

• biological source: Porcine pancreas
• Quality Segment: 200
• form: liquid
• technique(s): cell culture | insect: suitable
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.

Preparation Note

This formulation contains Tryptose (20 g/L); Dextrose (1 g/L); and NaCl (5g/L) adjusted to a final pH of 7.2.

Other Notes

This Tryptose broth is closely related to Tryptose phosphate broth, but does not contain the phosphate component and it contains a lower concentration of dextrose.

Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable