T1192

Anti-Thymine Dimer antibody, Mouse monoclonal

clone H3, purified from hybridoma cell culture

• Synonym(s): Mouse Anti-Thymine Dimer, Thymine Dimer Detection, Thymine Dimer Mouse Antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01865339
• Conjugate: unconjugated
• Clone: H3, monoclonal
• Application: DB, ELISA (c), ICC
• Citations: 7

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: H3, monoclonal
• form: buffered aqueous solution
• species reactivity: chicken, wide range
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): capture ELISA: suitable, dot blot: 0.5-1 μg/mL, immunocytochemistry: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

UV light induces the formation of thymine dimers that subsequently leads to DNA damage in cells. Monoclonal antibodies that bind to thymine dimers enable the study of cell death and UV-induced DNA damage during photocarcinogenesis . Monoclonal Anti-Thymine Dimer binds to the (5′-6′) cyclobutane type of homothymine or thyminecytosine heterodimers in single-stranded DNA. The antibody has a decreased affinity for the dimer in short oligonucleotides (an oligonucleotide of atleast 10-20 thymine residues is required for the labeling of the probes). The product facilitates the use of a sensitive and non-radioactive technique for the analysis of thymine dimerization.

Immunogen

tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.

Application

May be used in Southern blotting at 0.5-1μg/mL using UV induced thymine dimers of λ phage DNA fragments.

Monoclonal Anti-Thymine Dimer antibody is suitable for use in dot blot (0.5-1 mg/mL), immunocytochemistry (1:100) and capture ELISA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

Distributed under license.
Patents WO87/01134, EP 0233 177 B1

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable